Tinospora cordifolia (Willd) Miers ex Hook. F. & Thoms and Aerva lanata are medicinally important plants due to the presence of numerous secondary metabolites. The notable medicinal properties reported are antidiabetic, antiperiodic, antispasmodic, antimicrobial, antiinflammatory, anticancer, antiarthritic, antioxidant, hepatoprotective, immunomodulatory activities. Preliminary phytochemical screening and secondary metabolite analysis was reported in the present study. The qualitative analysis showed the presence of steroids, alkaloids, flavonoids, phenolics, saponins, tannins etc. Quantitative analysis reveals higher amount of phenols (172.33±2.52), tannins (186.67±5.21), alkaloids (520.33±9.61) and flavonoids (169.33±8.33) in T. cordifolia when compared to A. lanata.