The investigation of green chemistry approaches in medicinal chemistry has been prompted by the growing need for efficient and ecologically friendly chemical synthesis methods. These articles emphasize environmentally friendly pyrazole derivative synthesis techniques, which are good substitutes for synthetic techniques that uphold green principles. They also focus on Schiff bases, formed by condensing carbonyl compounds and primary amines, which have diverse industrial and biomedical applications, and their synthesis is being made more sustainable through green chemistry. In this study, we present the synthesis of (Z)-1,5-dimethyl-4-(((3-(3-nitrophenyl)-1-phenyl-1H-pyrazol-4-yl) methylene) amino)-2-phenyl-1,2-dihydro-3H-pyrazol-3-one(9a-9j). This was achieved through the condensation of 4-amino-1,5-dimethyl-2-phenyl-1,2-dihydro-3H-pyrazol-3-one with a 1,3-diphenyl-1H-pyrazole-4-carbaldehyde derivative, utilizing the green solvent 2-methyltetrahydrofuran. The characterization of all newly synthesized compounds was accomplished through spectral data analysis, including IR, 1H and 13C NMR spectroscopy, and mass spectrometry. The antimicrobial activity of the synthesized organic molecule derivatives was evaluated against a diverse array of microorganisms, including gram-positive and gram-negative bacteria and pathogenic fungi. To further investigate this, molecular docking studies were conducted to determine the binding affinity and interaction patterns of the synthesized compounds (9a-9j) with the crystal structure of the Staphylococcus aureus (3GR6) target protein.
