The current study aims to examine the clot dissolving, anticoagulant and antiplatelet, activities of Finger Millet Extract (FME). FME exhibited proteolytic activity as it hydrolyzed casein with the specific activity of 0.212units/mg/min. FME was analyzed for plasma re-calcification time using both human platelet rich and poor plasma. Interestingly, it showed strong anticoagulant effect in both PRP and PPP by enhancing the clotting time from control 210s to 660s and 246s to 780s respectively at the concentration of 12µg. FME in unambiguously prolonged the clot formation process of only APTT but not PT. Furthermore, FME dose dependently hydrolyzed only Aα and Bβ chains of human fibrinogen without affecting γ chain. In addition, it also dissolved human fibrin clot but not other plasma proteins. Furthermore, FME inhibited the agonists such as ADP and epinephrine induced platelet and it did not hydrolyze RBC cells suggesting its nontoxic properties.
