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In-vitro study on α-amylase inhibitory activity and phytochemical screening, test for inorganic elements, proximate analysis, qualitative and quantitative characterization of phytoconstituents of Mauritius papeda(Citrus hystrix) leaves having anti-diabeti

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Author: 
Kiran Kumar, S.J. and Deenadayalan, K.
Abstract: 

The present study was to evaluate preliminary photochemical analysis, inorganic elements and proximate analysis by leaves extract of Mauritius papeda by using solvent like methanol and water. Phytochemical analysis showed the presence carbohydrate, reducing sugars, phenolics, flavonoids, glycosides, saponnins and steroids. The moisture content of the crude sample was found to be 10.01 ± 1.40 while the water extractive index was 1.26 ± 0.0710. Quantitative analysis of phenols, flavonoids and Triterpenoids was further performed. Additionally inorganic elements like iron, chloride and sulphate were identified by total ash analysis. The same extract was used for quantitative determination of total flavonoid content (85.88 mg/g), total phenolic content (69.78 mg/g) and total triterpenoids content (09.22 mg/g).The methanolic extract had the highest antioxidant property compared to other fractions. α-amylase inhibitors are used in the treatment of type II diabetes mellitus. This study aims to identify the α-amylase inhibitors in Mauritius papeda (Citrus hystrix). Mauritius papeda leaves were subjected to sequential solvent extraction tested for α-amylase inhibition. The results indicates methanolic extracts exhibited highest α-amylase inhibitory activity with IC50 of 39.11±1.33 µg/ml. Pancreatic α-amylase inhibitors offer an effective strategy to lower the levels of post prandial hyperglycemia via control of starch breakdown. This finding could be used to support the use of these plants for treatment of diabetes and also it is an interesting phyto-chemicals to be developed as a new drug.

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