The Within reaction medium “Donators + membrane - redox potentials three - state line system + O2 + АDP + Pi + H+ + nH + membrane space = (ATP + heat energy) + H2O + nH + matrix + CO2”, which is belong to the the membrane redoxy potential three state dependent 9 stepped full cycle of proton conductance have been conducted such processes as the oxygen will then consume four protons from the matrix to form water, while another four protons are pumped into the IMS), resulting to form of proton gradient. The normal functioning of reaction medium “Donators + membrane - redox potentials three - state line system + O2 + АDP + Pi + H+ + nH + membrane space = (ATP + heat energy) + H2O + nH + matrix + CO2”, which is belong to the the membrane redoxy potential three state dependent 9 stepped full cycle of proton conductance is accompanied with these processes as protons and electrons are transferred from donors as fatty acids, glucose, and aminoacids, by using these complex reactions as Glycolysis, Oxidative deamination, Betta-oxidation followed by Krebs cycle, to electron acceptors such as oxygen in redox reactions, release the energy stored in the relatively weak double bond of O2, lead to formation of proton gradient, followed by oxidative phosphorylation, resulting to ATP synthesis. Within reaction medium “Donators + membrane - redox potentials three - state line system + O2 + АDP + Pi + H+ + nH + membrane space = (ATP + heat energy) + H2O + nH + matrix + CO2”, which is belong to the the membrane redoxy potential three state dependent 9 stepped full cycle of proton conductance the oxygen will then consume four protons from the matrix to form water while another four protons are pumped into the IMS) , resulting to form of proton gradient. ATP synthase, also called complex V, is the final enzyme in the oxidative phosphorylation pathway, uses the energy stored in a proton gradient across a membrane to drive the synthesis of ATP from ADP and phosphate (Pi), this is expressed in following equation, supposed by us as “Donators + membrane - redox potentials three - state line system + O2 + АDP + Pi + H+ + nH + membrane space = (ATP + heat energy) + H2O + nH + matrix + CO2”, which is belong to the the membrane redoxy potential three state dependent 9 stepped full cycle of proton conductance, described by us. The relationship between proton gradient generation and oxidative phosphorylation also have been appeared as the metabolic pathway in which cells use enzymes to oxidize nutrients as protons flow back across the membrane and down the potential energy gradient, through a large enzyme called ATP synthase with participation of process as chemiosmosis, the energy is tapped, the ATP synthase uses this energy to transform adenosine diphosphate (ADP) into adenosine triphosphate, named as phosphorylation reaction, which is drived by the proton flow, which forces the rotation of a part of the enzyme (ATP synthase is a rotary mechanical motor) within reaction medium as “Donators + membrane - redox potentials three - state line system + O2 + АDP + Pi + H+ + nH + membrane space = (ATP + heat energy) + H2O + nH + matrix + CO2”, which is belong to the the membrane redoxy potential three state dependent 9 stepped full cycle of proton conductance, described by us. Firstly we are reporting that the genertaion of proton gradients, preceded by oxidation of donators, which have been conducted in the left side of reaction medium expressed as “Donators + membrane - redox potentials three - state line system + O2 + АDP + Pi + H+ + nH + membrane space”, meanwhile, the process of phosphorylation have been occurred in the right side of reaction medium expressed as” (ATP + heat energy) + H2O + nH + matrix + CO2” (M.Ambaga, A.Tumen-Ulzii, 2019) followed by the formation of proton gradients.
