Among white rot basidiomycetes Pleurotus sp., is reported to be most efficient colonizers and degraders of lignocelluloses. The white rot edible mushroom Pleurotus platypus has considered as a moderate temperature loving fungus, having an optimum growth temperature ranges from 20-30°C. Alkaline pH (8) favored the fungal growth and extracellular lignin peroxidase and laccase production. Pleurotus platypus reported to produce extracellular lignin peroxidase under nitrogen and sulphur starvation. Among the nitrogen limiting condition tested, the organic rich yeast extract (1%) basal medium favored the production of lignin peroxidase (45.4 U/L) and in the absence of nitrogen favored the production of laccase (9300 U/L). The sulphur containing aminoacids cysteine and methionine comparatively reduced the ligninolytic enzymes production in all the basal media tested. The protein profile in SDS-PAGE gel showing band near 40 KDa and 60 KDa indicates the presence of lignin peroxidase and laccase in partially purified enzymes. Pleurotus platypus grown on sugarcane leaves and coir pith produced the extracellular lignin peroxidase and laccase.
