The study dealt with isolating the flavonoids from the rhizomes of curcuma sp. (Curcuma longa) and identification by the fast high performance liquid chromatographic (FHPLC).The results showed that the curcuma contained many kinds of the flavonoids (9.97μg/g of Curcumin, 8.07μg/g of Demethoxycurcumin, 39.49μg /g of Curcumol, 9.30μg/g of Isocurcumin and 8.54μg/g of Germacrone).The study is conducted to estimate the activity of the isolated Flavonoid extracts from curcuma as antioxidant in vitro by measuring the reducing power and their capacity of scavenging hydrogen peroxide as compared with the standard compound ascorbic acid. The results indicated that the Flavonoids had a reducing power and a capacity of scavenging hydrogen peroxide. This is observed through increasing the intensity of absorbance with increasing the concentration. The experimental study is done to evaluate the activity of the flavonoid extract as antioxidant in vivo on the local male rabbits treated with ethanol (5%) freely in potable water. The experimental animals are divided into five groups, each group has six rabbits.(C1)The first control group is given only normal water, (C2) the second control group is given water containing (5%) ethanol. [Groups G1, G2 and G3 were given 4,8 and 12 mg flavonoid per kg body weight, dissolved in 5% ethanol respectively for four weeks].Certain antioxidants are measured as Glutathione Peroxidase GPX, Glutathione and Uric acid.The results indicated there is a significant increment at levels of the GPX and GSH in all groups except the group (G1) as compared with the control group (C2).Whereas the Uric acid had a significant decrease in all group as compared with the control group (C2).
