Effects of leptadenia hastata extract on artemia salina and dose rate on organs of ulcer induced rats

Leptadenia hastata is often used in traditional medicine and it is generally agreed that medicinal plants and their products are naturally safer than their synthetic counterpart drugs. In the present study, Leptadenia hastata; a crawling perennial plant was used to determine the cytotocity and the effect of increasing dose rates of five different concentration of hexane extracts on organ weights of ulcer induced rats. A total of 40 white albino rats (male sexes) weighing 150-190 g/kg/bwt of two-month-oldand brine shrimps were used in this study. The rats were stabilized for 1 week in their separate cages of 5 rats each of eight groups A, B, C, D E,F, G and H; an increasing dose rates of aqueous extract of Leptadenia hastata viz., 100 200, 300, 400 and 500mg/kg bwt were administered orally daily for 20 days to groups C, D, E, F, G and H respectively. Control group A was given normal saline, B (negative control) was given Indomethacin while groups C (Positive control) Omeprazole for the same experimental period. The rats were sacrificed on day 21 and organ weights were measured using Mettler balance. Cytotocity was evaluated in terms of LC50 (Lethality concentration) ten nauplii were added into three replicate of each concentration of the extract. After 24hrs the surviving shrimp larvae were counted and LC50 was assessed. The results of the organs revealed a slight change in the mean weight of some organs (1.51±0.01 brain) with little or no significant treat with increase in concertation of the extracts when compared with the control (1.50±0.03). While the cytotoxicity potent of the hexane extract exhibited low potent activity with LC50 values9421.49µg/mL.