Development Of Tio2-Peg-Ptx Nanoparticle Based Drug Systems And Investigation Of Anticancer Activity On Sh-Sy5y Cells

The use of nanoparticles in biomedical and bioengineering fields has revolutionized cancer treatment. In this study, we aimed to investigate the anticancer activity of the nanotechnologically produced TiO2-PEG-PTX drug on SH-SY5Y neuroblastoma cell lines. Our study, TiO2 nanoparticles used were synthesized, coated with PEG, and PEG-TiO2 nanostructure system was loaded with PTX.UV analyse of suspensions prepared at different concentrations of TiO2, PEG-TiO2, PTX, and PEG-TiO2-PTX nanostructured system were performed. The synthesized drugs were performed to the SH-SY5Y neuroblastoma cell line and anticancer activity of these drugs were determined by using MTT method. The SH-SY5Y cells were treated with different concentrations of TiO2 (5-100 μg/ml) for 24, 48 and 72 hours. The effects of these drugs on the SH-SY5Y cells were compared with the control group and IC50 values were determined for 24, 48 and 72 hours. In this study, it was shown that the effect of TiO2-PEG-PTX nanocarrier system on SH-SY5Y cells was inhibitory to growth in cancer cells when compared with control group and PTX.