Analysis of bioactive compounds from single herbal siddha distillate “vasambu dravagam” through gc-ms

Background:Theeneer, the hydro distillate formulations of Siddha medicine is getting popularity and wide clinical acceptance owing to its faster therapeutic outcome. The raw drugs single or compound is preprocessed and subjected to distillation by following the traditional parameters mentioned in the siddha texts. Vasambu (Acoruscalamus Linn.) is a well known herb that has very reputed use in Traditional system like Siddha medicine and other Ethno medicinal practices. The rhizome part is distilled for its medicinal purposes and is termed as Vasambu Dravagam. The drug is indicated in siddha medicine for correcting the vitiations of three humors namely Vazhi, azhal and Iyyam (Vatham, pitham and Kapham). Objectives: The present study aims in preparing the distillate of Acoruscalamus rhizome and to analyze the drug with GC-MS (Gas chromatography Mass Spectrometry). Methods: The Rhizome part of Vasambu (Acoruscalamus Linn.) was distilled after following the preprocedures and further the distillate were analyzed for screening the active components. Results: Vasambu Theeneer was colorless with slight peculiar nauseating odor, and having mild sweet and pungent taste.Through GC-MS a total of 4 compounds were identified from Acorus distillate. Predominant peak belonging to 4-[4-(1-Phenyl-1H-1, 2, 4-triazol-5-yl)-1, 3-thiazol-2-yl] pyridine then asarone, Silicic acid and 2-(2-Benzothiazolyl)-3-[4-(dimethylamino) phenyl] acrylonitrile. Discussion: Asarone and the volatile siloxane silicic acid is the active component of this Acorus distillate which has reputed Pharmacological activity in deciding the outcome of the drug.. Conclusion:Vasambu Dravagam and its possibility in clinical aspects for broad spectrum of diseases may be supported with this preliminary screening and interpretations.